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Image Search Results
Journal: BMC Cardiovascular Disorders
Article Title: The rat STSL locus: characterization, chromosomal assignment, and genetic variations in sitosterolemic hypertensive rats
doi: 10.1186/1471-2261-3-4
Figure Lengend Snippet: Alternative splicing of Abcg8 mRNA expression in SD, SHR and WKY rats by RT-PCR Panel A shows the gene organisation of Abcg8 , and below it the organisation of an alternatively spliced form detected by sequencing clones from a rat intestinal cDNA library (see Text). Panel B shows that the effect of the inclusion of exon 2b in the mRNA results in an in-frame addition of 22 amino acids. Total RNAs were prepared from liver and small intestine from SD, SHR and WKY rats maintained on a standard rodent chow diet and analysed by RT-PCR with primers (see Methods) based outside the alternatively spliced exon 2b. Direct amplification of a pooled cDNA library sample indicated a major DNA product of 396 bp, with a minor product of 462 bp, containing exon 2b. DNA products, using cloned wild type Abcg8 cDNA (wt) and alternatively spliced cDNA (AS) are shown in tracks 3 and 4 respectively. As can be seen, liver and intestine RNA from all three strains show a minor DNA product of 462 bp (confirmed by sequencing) contained the insertion of exon2b sequences.
Article Snippet: Rat cDNAs for Abcg5 and Abcg8 were identified previously by amplification of these sequences from a
Techniques: Alternative Splicing, Expressing, Reverse Transcription Polymerase Chain Reaction, Sequencing, Clone Assay, cDNA Library Assay, Amplification
Journal: BMC Cardiovascular Disorders
Article Title: The rat STSL locus: characterization, chromosomal assignment, and genetic variations in sitosterolemic hypertensive rats
doi: 10.1186/1471-2261-3-4
Figure Lengend Snippet: Protein Expression of Abcg5 in liver total membrane preparations. Membrane preparations (20 μg protein) from rat tissues were isolated and analysed by SDS-PAGE, blotted and probed with anti- Abcg5 antiserum (R116, panel A), probed with anti-transferrin antiserum (panel B) or the gel was fixed and stained with Coomassie Brilliant Blue R-250 (panel C). Lane 1 contains a recombinant E. coli expressed N-terminal fragment of sterolin-1 as a control for antibody binding, lanes 2 and 3 contain Sprague-Dawley (SD) spleen membrane extracts. Liver from Wistar (lanes 4 and 5), SHR (lanes 6 and 7) and Sprague-Dawley (SD, lanes 8 and 9) Densitometry measurements when normalised to transferrin show a 2-fold increase in sterolin-1 protein in SHR rat liver samples, compared to SD and Wistar rat liver. No antibody for sterolin-2 is currently available.
Article Snippet: Rat cDNAs for Abcg5 and Abcg8 were identified previously by amplification of these sequences from a
Techniques: Expressing, Membrane, Isolation, SDS Page, Staining, Recombinant, Control, Binding Assay